| 论文题目: |
Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library |
| 论文题目英文: |
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| 论文编号: |
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| 第一作者: |
夏正俊 |
| 第一作者英文: |
Xia, Z. J. |
| 刊物名称: |
Analytical Biochemistry |
| 卷期页码: |
, 444, 38-40 |
| 发表年度: |
2014 |
| 联系作者: |
夏正俊 |
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| 摘要: |
Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.
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