| 论文题目: |
Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time |
| 论文题目英文: |
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| 论文编号: |
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| 第一作者: |
Li, Zhaobo |
| 第一作者英文: |
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| 刊物名称: |
Crop Journal |
| 卷期页码: |
9, 4, 767-776 |
| 发表年度: |
2021 |
| 联系作者: |
孔凡江 |
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| 影响因子: |
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| 参与作者: |
Z. B. Li, Q. Cheng, Z. R. Gan, Z. H. Hou, Y. H. Zhang, Y. L. Li, H. Y. Li, H. Y. Nan, C. Yang, L. N. Chen, S. J. Lu, W. Q. Shi, L. Y. Chen, Y. P. Wang, C. Fang, L. |
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| 摘要: |
Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of soybean adaptation to diverse latitudes is still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2 (LNK2) homeologs of Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs were designed for editing the four LNK2 genes. A transgene-free homozygous quadruple mutant of the LNK2 genes was developed using the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9). Under long-day (LD) conditions, the quadruple mutant flowered significantly earlier than the wild-type (WT). Quantitative real-time PCR (qRT-PCR) revealed that transcript levels of LNK2 were significantly lower in the quadruple mutant than in the WT under LD conditions. LNK2 promoted the expression of the legume-specific El gene and repressed the expression of FT2a. Genetic markers were developed to identify LNK2 mutants for soybean breeding. These results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four LNK2 genes shortens flowering time in soybean. Our findings identify novel components in flowering-time control in soybean and may be beneficial for further soybean breeding in high-latitude environments. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. |
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